titer(titre)雙語(yǔ)例句
1. The ELISA titre was 1:2000.By cell fusion, 46 hybridoma cell lines were screened, and 10 lines were cloned with limited dilution method.16 lines secreting anti-bFGF monoclonal antibody were been developed, and 2 lines targeted fusion protein. Sensitive ELISA and dot-ELISA for bFGF was developed with this mAb. The detection limit of them were 0.1 ng/well and 0.5 ng/well. The expression level of anti-bFGF mAb by different rebuilt engineering cells were identified by western blot and to direct rebuild recombiment engineering cell. The dose and character of anti-bFGF mAb inhibiting bFGF biology activity were searched by 3T3 cell line. Searching 20 tissue of liver cancer, liver cancer cell lines and general tissue of liver, finding bFGF were highly expressed in tissues of liver cancer and liver cancer cell lines. Affinity chromatography purifying bFGF was set up by mAb binging CNBr-pepharose 4B, and the purification was 95%. We found that the titer of anti-bFGF antibody was very high in serum of neuropathic amyotrophia.
應用細胞融合制備46株雜交瘤，對其中10株進(jìn)行克隆化，獲得bFGF特異單抗16株，2株針對融合蛋白；應用該單抗建立了0.1ng/孔靈敏度的ELISA，0.5ng/孔敏度的斑點(diǎn)ELISA；用Western-blotting鑒別了經(jīng)改造不同工程菌蛋白表達，指導重組工程菌改造；用3T3細胞培養研究了單抗抑制bFGF生物學(xué)活性的劑量和特點(diǎn)；合作研究了20例肝癌、肝癌細胞株和正常肝組織，發(fā)現前者bFGF高表達；應用單抗偶聯(lián)CNBr-sepharose 4B建立了小量免疫親和層析純化bFGF，純度達到95%；發(fā)現神經(jīng)性肌萎縮患者血清中含有高滴度的bFGF抗體，已有10多家單位引用單抗或進(jìn)行合作。
2. Diazotization was used to conjugate sulfadimethoxine to carrier protein BSA and obtained immunizing antigen. The coating antigen OVA-SDM was obtained in the same way, ultravioletand SDS-PAGE were used to identify SDM artificial antigen. BALB/C mice were immunized with BSA-SDM, the titre of polyclonal antibody was detemined by indirect ELISA and blocking ELISA. The hybridoma lines that secrete SDM mAb were established with using monoclonal antibody hybridoma technology. The immunological traits such as titer, affinity, sensitivity and specificity of the mAb were characterized.
用重氮化法將SDM偶聯(lián)于載體蛋白BSA和OVA，合成免疫原BSA-SDM和包被原OVA-SDM，并用紫外掃描、SDS-PAGE進(jìn)行鑒定；用BSA-SDM免疫BALB/C小鼠，間接ELISA和阻斷ELISA選擇細胞融合備用鼠；應用雜交瘤技術(shù)建立分泌SDM mAb細胞株，用體內誘生腹水法制備SDM mAb；對SDM mAb的效價(jià)、親和性、敏感性和特異性等免疫學(xué)特性進(jìn)行鑒定。
3. The recombinant adenovirus of Ad-Cp-CDglyTK was packaged, amplified and purified in 293 cells, and the virus titre was determined by TCID50 method. The CDglyTK gene expression in CNE1 and NP69 were examined by reverse transcription-polymerase chain reaction after in vitro transfection in CNE1 and NP69 cells. The killing effect of Ad-Cp-CDglyTK/GCV+5-FC on CNE1 cells wasdetected by MTT method. RESULTS:The results of restriction enzyme digestion and DNA sequencing showed that the tk, cd, and Cp gene were inserted into the pDC316 plasmid in correct orientations. The titer of the recombinant adenovirus was 5.6×1012 TCID 50/L. The Cp fragment was amplified from the total RNA of the transfected CNE1 cells by RT-PCR.
結果：經(jīng)DNA測序、限制性酶切法分析顯示pDC316-Cp-CDglyTK含完整正確的tk、cd、Cp基因序列，在293細胞中包裝擴增后病毒滴度為5.6×1012 TCID50/L，體外轉染鼻咽癌CNE1細胞株與正常鼻咽NP69細胞株后，采用RT-PCR法從CNE1細胞株總RNA中擴出Cp片段，而NP69細胞株未檢測到相應基因mRNA表達，MTT結果顯示經(jīng)前體藥物處理轉染后CNE1細胞株與NP69細胞株，5-FC+GCV聯(lián)合用藥較單一前體藥物對CNE1細胞具有更強的抑制作用（P＜0.05），聯(lián)合用藥對NP69細胞株未見(jiàn)明顯殺傷作用。
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